human embryonic kidney cell line hek 293t (ATCC)

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ATCC human embryonic kidney cell line hek 293t
Human Embryonic Kidney Cell Line Hek 293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+kidney+hek+293t+cells/293T/pm42288668-42-12-21
Average 99 stars, based on 37978 article reviews

human embryonic kidney cell line hek 293t - by Bioz Stars, 2026-09

99/100 stars

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Expressing:

Article Title: Autism-linked protein POSH balances NMDAR signaling via a self-limiting kinase-scaffold loop
Article Snippet: The medium was changed to the serum-free Neurobasal (Gibco) medium supplemented with 2% B27 (Gibco) supplement and GlutaMAX (Gibco) 1 h after plating and was half replaced with fresh medium every 4 days. .. Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for heterologous expression and biochemical experiments. ..

Cell Culture:

Article Title: Extracellular vesicles from hypertrophic cardiomyocytes promote breast cancer progression.
Article Snippet: .. Cell lines, cell culture, and transfection The human MDA-MB-231 and BT549 breast cancer cell lines, the human cardiomyocyte AC16 cell line, hiPSC-cardiomyocytes, and human embryonic kidney (HEK) 293T cells were purchased from ATCC and were cultured in-house. .. MDA-MB-231, BT549, and HEK-293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Australia) while AC16 cells and hiPSC-cardiomyocytes were cultured in DMEM/F-12 (Gibco).

Article Title: Type II tRNA cleavage by SLFN14 endoribonuclease variants linked to inherited thrombocytopenia drives global translational repression.
Article Snippet: Additional reagents included high-range RNA ladder (ThermoFisher, SM1821) and the Low Range ssRNA Ladder (NEB, N0364S). .. Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 °C with 5.0% CO 2 . ..

Article Title: Modular mRNA platform for in vivo antigen-specific immune tolerance
Article Snippet: .. Human embryonic kidney (HEK)-293T cells (ATCC, Cat# CRL-3216) and Lenti-X cells (Takara, 632180) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, 08458-16) supplemented with 10% heat-inactivated fetal calf serum (FCS; Thermo Fisher Scientific, A5256701), 100 U/mL penicillin, and 100 U/mL streptomycin (FUJIFILM Wako, 16823191). .. Murine lymphocytes were cultured in RPMI (Nacalai Tesque, 30264-56) supplemented with 10% FCS, 1× non-essential amino acid (FUJIFILM Wako, 139-15651), 1 nmol/L sodium pyruvate (Nacalai Tesque), 100 U/mL penicillin, 100 U/mL streptomycin, and 0.05 μM 2-mercaptoethanol (Thermo Fisher Scientific, 21985023).

Transfection:

Multiple Displacement Amplification:

Modification:

Article Title: The Q226H Mutation in Avian H5N1 Hemagglutinin Mediates a Path towards Structural Adaptation in Humans
Article Snippet: .. Human embryonic kidney (HEK) 293T cells were obtained from the American Type Culture Collection (ATCC) and maintained in D10 media (Dulbecco’s Modified Eagle Medium [DMEM; Gibco] supplemented with 10% heat-inactivated fetal bovine serum [FBS; Gibco]) at 37°C and 5% CO2. .. Expi293F cells (Life Technologies), used for mammalian expression of recombinant Abs and hemagglutinin antigens, were maintained in Expi293F expression medium (Life Technologies) at 37°C and 8% CO2.

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